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Purified water

Front Mol Biosci (2022) 9:945917

There's no one-size-fits-all number

FACS is not sufficiently accurate to distinguish particles smaller than 0.5 m, but EXOs are typically between 30 and 120 nm in diameter (5), making it difficult to determine their dimensions ( Other methods for analyzing exosome particle size and shape include scanning electron microscopy (SEM) and transmission electron microscopy (Sharma et al., 2023)