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139 Liu WZ, Li XY, Bing XZ, Yu HN, Pan L, Li J, et al

Its clinical efficiency is still dubious

Formans team

The assay used specific primers that were designed to target the crossover region between the deleted genes and the AES and MAP genome of the respective essential gene

For western blot, samples were loaded onto 4-12% bis tris gels (Thermo Fisher #NW04120) and separated by electrophoresis at 165 volts for 60-90 minutes