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Samples were then evaporated to dryness in Speed vac and resuspended in 500 l Buffer A (95% H 2 O, 5% CH 3 CN, 0.1% FA) with sonication and loaded on HPLC for offline high pH fractionation following conditions adapted from previous work 57

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Hvid-Jensen, F., Pedersen, L., Drewes, A

In fatty liver disease specifically, elevated B12 would more likely occur in advanced stages with significant liver damage rather than in simple steatosis

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