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BBGC (200 mg/kg) treatment was intraperitoneally administered on days 0 and 8, and trabectedin (0.100 mg/kg) was intravenously injected on days 0 and 7

The accumulation of pathogenic microorganisms is one of the reasons for poor digestion in aquatic organisms

Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation and monocytes were isolated by plastic adherence by culturing 1 10 6 cells/mL for 2 h in serum-free media at 37 C [20]

The advent of single-cell RNA sequencing (scRNA-seq) and related multi-omic technologies has transformed this landscape by enabling high-resolution dissection of MuSC states, fate transitions, and interactions with the niche

These studies allow us to refine the formulation iteratively, ensuring that the liposomes meet functional requirements before scale-up