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After incubation, the membranes were washed three times and then incubated at room temperature for 1 h with secondary antibodies, including IRDye 680RD goat anti-mouse IgG (H + L) (Licor, 926-68070), IRDye 680RD goat anti-rabbit IgG (H + L) (Licor, 926-68071), IRDye 800CW goat anti-mouse IgG (H + L) (Licor, 926-32210), IRDye 800CW goat anti-rabbit IgG (H + L) (Licor, 926-32211)

These models don't predict the future

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The RDA for Vitamin B12 for adults is set to 2.4 mcg (2.6 mcg in pregnancy and 2.8 mcg while breastfeeding) and represents the amount needed to prevent a damaging Vitamin B12 deficiency

Comparison of 13 C and 13 CO secondary chemical shifts indicates weak consensus -strand propensity (consecutive negative C and CO values) for N-terminal half residues Glu1258-Asp1260 in both the wild-type and mutant S tails (Fig