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Without suppressor, the p-hydroxybenzoic acid eluent used in this application, resulted in negative peaks that were difficult to quantify accurately

MS/MS data were extracted and searched against in-house Mascot Server (Revision 2.5.0), a search engine that uses mass spectrometry data to identify and characterize proteins from sequence databases

However, neither molecular mechanisms responsible for the APE/Ref-1 gene expression induction upon oxidative injury nor functional data regarding the real in vivo role played by post-translational modifications in controlling APE/Ref-1 functional activities are clear.24 Understanding the possible relationships existing between APE/Ref-1 levels and oxidative-stress-based human liver pathologies is of enormous importance, not only for the comprehension of the role and mechanism that APE/Ref-1 may play in the initiation and development of these pathologies but also for developing diagnostic markers and therapeutic tools

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Ribonuclease A is remarkably stable