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17 The HPA axis is the most important neuroendocrine stress system, and the cortisol awakening response (CAR) is a core biomarker of HPA axis regulation

HFD high-fat diet mice in the vehicle cohort received three subcutaneous (SC) saline (1 ml/kg) injections/day ( 0930, 1330, 1730 h) and mice in the treatment cohort received three SC injections of the NNMT inhibitor 5-amino-1MQ at a dose of 20 mg/kg/injection for a total dose of 34 mg/kg/day of the parent compound (calculated according to free weight) for 11 days

Results depend on the formulation, concentration, consistency of use, and individual skin characteristics

For non-parametric data, Mann-Whitney or Kruskal-Wallis tests were performed

These include intermittent fasting, or fasting for certain hours of the day or certain days of the week, and fasting mimicking fasting for a few consecutive days per month