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The cells were left in the treatment medium for 24 h after which they were washed twice and collected for the luciferase assay, which was performed according manufacturers instructions with a kit from Promega Corporation (Madison, WI, USA)

BIOMAX OxiTec Glutathione(GSH/GSSG/Total) Assay Kit Sample Total GSH GSSG,GSH

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Key Takeaways There is no single peptide timeline: each peptide targets a different biological system and produces results on a different schedule

Furthermore, the effect of NFX on other cancer-promoting pathways was explored