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10.1007/s12035-020-02128-5 229 YangW.Tiffany-CastiglioniE.KohH

Tissue ROS staining To measure the ROS level of cardiac tissue, heart was embedded with optimal cutting temperature compound and placed in the refrigerator at 80C, the frozen heart was cut into 6 m slices and washed them with cold PBS

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The less pronounced effect observed in this group may be attributed to the specific properties of the CBD-rich formulation (SRD + CBD), which, while exhibiting potent antioxidant and anti-inflammatory effects, it may not influence the key pathways of lipid metabolism as effectively as other formulations

SensoLyte 520 TACE Activity Assay Kit uses a 5-FAM (fluorophore) and QXL 520 (quencher) labeled FRET peptide substrate for continuous measurement of enzyme activity