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The peptide sequence Gly-His-Lys chelates a single Cu(II) ion through the histidine imidazole nitrogen and the deprotonated amide nitrogens of the backbone

Cushing, V

When these receptors activate, fat cells release stored lipids for energy use

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Briefly, samples of WT and AS-treated NPCs and their respective vehicle NPC groups (1 10 6 cells per sample) were prepared with mammalian cell lysis buffer (abcam ab179835) and deproteinizing sample preparation kit -TCA (abcam ab204708) according to the manufacturers instructions