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For researchers building a comprehensive IGF axis research workflow, the standard approach is to use IGF-1 LR3 as the primary tool for sustained IGF1R activation studies, native IGF-1 for short-pulse studies where IGFBP biology is itself the research question, and DES IGF-1 for the narrow set of experiments requiring receptor activation with rapid washout

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While surgical denervation does not adversely affect expression of Mist1 (Fig 3B and E), muscarinic stimulation is sufficient to increase MIST1 in human cultures and suggests that although not required for acinar cell identity, acetylcholine/muscarinic signaling may act as a positive regulator of the secretory program