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Further studies are needed to define the best Se-status biomarkers related to male fertility, their optimal ranges, analytical techniques for their assessment, and the optimal Se supplementation dosages, formulations, and treatment durations

Thereafter, the membrane underwent a 1 h blockade with 5% skim milk and an overnight incubation with primary antibodies rabbit anti-NLRP3 antibody (1:1,000, ab270449, Abcam), rabbit anti-GSDMD-N antibody (1:1,000, DF12275, Affinity Biosciences), rabbit anti-cleaved caspase-1 antibody (1:500, ab32042, Abcam), and rabbit anti-apoptosis-associated speck-like protein (ASC) antibody (1:500, AB3607, Sigma-Aldrich) at 4C, followed by washes with Tris-buffered saline with Tween 20

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Given that the portal venous system of the liver collects blood from the intestines, there exists a complex interdependent relationship between these two organs known as the gut-liver axis [27]

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