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He S, Cao Y, Xie P, Dong G, Zhang L

Some studies also show that meeting the recommended dietary intake may even potentially reduce the risk of miscarriage, but more data is still needed

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Then, the cells were incubated with a blocking solution containing 10% goat serum and 0.2% BSA (Biological Industries Israel Beit Haemek # 030-010-1B) in PBS with 0.1% Triton X-100, for 1 h at RT, followed by primary antibody incubation with anti-Nestin (anti-rat 1:250, abcam ab81462) at 4 C overnight