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10.3390/ijms21186462 8

Then switch to a 5mg vial for weeks 5-8 at 0.5mg weekly (2mg used, 3mg wasted)

Immunoprecipitation and Western blot HEK293T cells were collected and lysed in lysis buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Triton X-100, 10% glycerol, protease inhibitors and benzonase) and incubated on ice for 30 min

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