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When residues L43, W53, and F54 were substituted with alanine, the inhibitory ability was significantly reduced, indicating that these hydrophobic residues were essential for FOXO4 FHD binding (Table 1)

These molecules have been shown or predicted to have therapeutic potential for numerous pathologies including dementia, neurodegenerative disease, diabetes and metabolic syndrome, cancer, and defective wound healing

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After washing 3 20min with 1ml 0,2% gelatin-TBS secondary antibody incubation occurred as described for the primary antibody and the coverslips were incubated for one hour at room temperature