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The isolated RNA was used in quantitative reverse transcription PCR (RT-qPCR) to assess expression of genes listed in Supplementary Table 1

(7) In the research I cited at the top of this article, doctors at the Italian Scientific Spine Institute in Milan wrote: We cannot conclude on the basis of this review whether surgical or nonsurgical treatment is better for individuals with lumbar spinal stenosis

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2) The study performed by Li et al employed a multi-pronged experimental design, integrating human tissue analysis, in vitro cell culture, and in vivo animal models to comprehensively investigate the role of FOXO4 in age-related male fertility and the therapeutic potential of FOXO4-DRI