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Pellets were resuspended in DMEM/F-12 medium (Life Technologies, Carlsbad, CA, USA) supplemented with 20% foetal bovine serum (FBS, Pan Biotech, Aidenbach, Germany) and penicillin/streptomycin (Life Technologies) and plated on 10 cm cell-culture dishes

In fact, the medical antidote for paracetamol overdose (N-acetylcysteine) works by replenishing glutathione stores (Prescott, 1983)

Thus, it is possible that AKT signaling pathway might play the regulatory role in HEM-induced stimulation of OL maturation

Insist on detailed Certificates of Analysis (CoAs) for specific batches, which should verify purity (e.g., via HPLC), heavy metal limits (Pb, As, Cd, Hg), and microbial contamination against Japanese Pharmacopeia (JP) or other international standards (USP, EP)