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Dec 2019;170:113662

Determination of -GCS activity: A 3 mL reaction system was prepared, including 0.1 mL of the crude enzyme solution sample, 100 mmol/L Tris-HCl with 20 mmol/L sodium glutamate, 10 mmol/L cysteine, 5 mmol/L adenosine triphosphate disodium, 100 mmol/L NaCl, 20 mmol/L magnesium chloride, and an appropriate amount of crude enzyme solution

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