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Kidneys were homogenized to measure the levels of ROS (Solarbio, Beijing, China), GSH (Solarbio, Beijing, China), and iron content (Jian Cheng, Nanjing, China) according to manufacturers instructions

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M1 macrophages play a key role in the initial stages of the inflammatory response by releasing considerable amounts of inflammatory agents such as tumor necrosis factor (TNF-), IL-6 and IL-12, which contribute to aggravating inflammation

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