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10.1001/jamanetworkopen.2020.24335 132 ShadkamiF.JonesA

The trial was permitted by Erzincan Binali Yldirim University Local Animal Ethics Committee (Meeting date: 26.09.2024, Meeting No: 09)

Jan 21, 2026 At the molecular level, IGF-1 LR3 represents one of the most significant modifications ever made to a growth factor

doi: 10.1517/14728222.12.1.19

the lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2 reducing Laemmli buffer and heated for ten minutes at 95 C