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4E), but not PlexinB3 (Fig

PMID: 31778746

He Y, Ji Z, Gong Y, Fan L, Xu P, Chen X, et al

Zain, M

Generally, protein purification entails five essential steps, i.e., extraction from biological matter, separation from lipids and nucleic acids (non-protein components), precipitation step, recovery of bulk protein from raw extract, utilization of hydrophobic or ion-exchange chromatography column for the separation of target protein from bulk protein, and more refined steps including affinity matrix to obtain highly refined target protein with high yields