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For the purification of protein samples used in the Octet experiments, an additional gel filtration step on a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in 20 mM Tris-HCl, pH 7.5 and 150 mM NaCl (TBS), was included

There is unmistakable preclinical and clinical evidence that the intensity, kind, and duration of ROS exposure all affect how much harm is caused by them