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TB-500 is often administered in cycles that involve a loading phase followed by a maintenance phase

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In parallel, Per2-luciferase rhythms were monitored in synchronized cells cultured in DMEM containing 0.1 mM luciferin using a LumiCycle apparatus (Actimetrics)

However, their molecular nature is still elusive (Bachhawat et al., 2013)

Moonens et al., 2018)