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Larish often suggests that patients wait for a period of time to see if the condition resolves, and if the condition is mild, then no treatment may be necessary

For immunofluorescence staining, cells were rinsed three times with PBS before being incubated with BODIPY TM 581/591 C11 dye in serum-free medium at 37 C with 5% CO2 for 30 min before being visualized under a fluorescence microscope (Evos FL, Thermo Fischer Scientific) with objective 40

Pretreatment of cultured preadipocytes with arachidonic acid during the differentiation phase without a cAMP-elevating agent enhances fat storage after the maturation phase