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Splicing and alternative splicing during pre-mRNA processing Pre-mRNA splicing is a crucial gene expression process in the cell nucleus, where introns (noncoding regions) are removed and exons (coding regions) are joined to form mRNA

After the incubation time with the probes, the cells were washed with PBS twice, incubated in complete medium and monitored in the Leica SP8 microscope equipped with a Lightning super resolution, CO 2 and temperature control module, 3 ultrasensitive hybrid detectors (HyD) to avoid phototoxicity and photobleaching