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Methods: We identified a MASLD cohort between 2007 and 2009 (baseline period), applying the validated algorithm (PMID: 30144434) to the VA EHR and selecting patients with at least one metabolic feature at baseline

Importantly, all the leaf samples for RNA-seq analysis were from the same leaf sample pools as the widely targeted metabolic study, ensuring the high quality of the dataset for integrated metabolic and transcriptomic studies

10.1523/jneurosci.4255-14.2015 255 PforteC.Henrich-NoackP.BaldaufK.ReymannK

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This is significant because age-related sleep disruption often involves not just melatonin deficiency but broader circadian gene dysfunction