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An Interleukin-23-Interleukin-22 axis regulates intestinal microbial homeostasis to protect from diet-induced atherosclerosis

Constructions expressing the Wild type PKCI (Wt PKCI) and PKCI containing a deletion of the first thirty amino acids of the N-terminus, including the pseudo-substrate (NPS PKCI) were both cloned in the mammalian expression vector pcDNA 3.1 in Bam HI and Xho I sites using the following primers: forward 5 ATATTTAGGATCCCAGATGGCTGACCCG 3/reverse 5 CAGGTCGGTCTCGAGCTACACATTAATGAC 3 and forward 5 A TATTTAGGATCCCAGATGAACGTGCACGAG 3 reverse 5 CAGGTCGGTCTCGAGCTACACATTAATGAC 3, respectively

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Rarer issues include infection, uneven reduction or an allergic reaction, which is why follow-up access to your clinician matters

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