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The synthesis method of the Cagrilintide comprises the third step of preparing 200mL of cracking reagent, adding the full-protection peptide under an ice bath condition, returning to room temperature after 0.5h, continuing to react for 2h, adding anhydrous ether for precipitation after the reaction is finished, performing centrifugal precipitation for multiple times, adding 300mL of ether each time, and drying to obtain the Cagrilintide product

K., Malmstrm, J., Hjlys-Larsen, K

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4 and 23, the amplitude of signaling reads where [ R ] ss can be expressed in terms of R o by rewriting Eq

Based on these observations, we designed peptides in which the individual hydrophobic residues were substituted with alanine