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The slides were conjugated with primary antibodies for 30 min at room temperature, followed by antibody detection using Dako REAL Envision detection system (Agilent Dako, Carpinteria, CA, USA)

Femmes enceintes et allaitantes : bien que les besoins soient accrus, environ 4,5 5 g par jour , la supplmentation doit rester dans les limites nutritionnelles recommandes pour viter tout dsquilibre mtabolique chez le ftus ou le nourrisson