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5c, compared with 5.5 nm for the measurement of F-actin as a control), a diameter of 37.8 nm (Fig

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Cellular senescence was assessed by SA--galactosidase staining (SA--gal staining) using senescence -galactosidase Staining Kit (Cell Signaling Technology, Beverly, MA, United States) according to the manufacturers instructions

Its administration resulted in improved behavioral scores, reduced visceral hypersensitivity, and decreased intestinal inflammation, suggesting that triptolide modulates the gut-brain axis and inflammation in tandem