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The analysis of neuron density further supports these findings, with the model group showing a marked reduction in neuron density (Figure 8C), reflecting the adverse effects of sleep deprivation on neuronal health

While some therapies may require a shorter duration for administration, others with more comprehensive nutrient blends or therapeutic goals may necessitate a longer session

Culture of isolated adult neurons was as previously described [19]

Following incubation with primary antibodies and rinsing, the blots were incubated for 1 h at room temperature with HRP-conjugated secondary antibody, according to the manufacturers recommended ratio