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ITC reveals no detectable change in global heat observed for selected other ligands, even for L -hercynine which binds weakly despite lacking only the thione sulfur atom of ET (Supplementary Fig

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Re-using a needle for both bac water draw and peptide injection Failing to swab the vial stopper with 70% isopropyl before each puncture Using a needle that's been left exposed to air for more than a few seconds Re-entering a peptide vial with the same needle you used to inject yourself (back-contamination) This is the lowest-incidence cause on the list most peptide buyers practice clean technique

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