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Furthermore, disease-related phenotypes were not observed in transgenic mice expressing human SOD1 that has multiple mutations including those at copper and zinc binding sites (H46R/H48Q/H63G/H71R/H80R/H120G) and two free Cys residues (C6G/C111S) with an ALS-linked mutation, H43R, and co-expression of wild-type human SOD1 did not cause the disease [35]

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Organ harvesting Mice were euthanised by cervical dislocation and tissues were flash-frozen in liquid nitrogen

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Athletes and fitness enthusiasts often utilize Vitamin B shots as a part of their fitness regimen to sustain energy, reduce fatigue, and improve overall metabolic health