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Transfected cells were grown at 37 C in 5% CO 2 in DMEM high glucose GlutaMAX (Gibco-Life Technologies) supplemented with 1% penicillin/streptomycin (Sigma Aldrich) and 5% of heat-inactivated fetal bovine serum (PAA, GE Healthcare Life Sciences) and selected with 0.2 mg

If more than five days have passed, its typically best to skip that dose and pick back up on your regular schedule the following week

doi: 10.1016/j.acthis.2013.11.012 155 ChangJWChoiJWLeeBHParkJKShinYSOhY-Tet al

You can also see a derm to get a prescription for clindamycin, a topical antibiotic, or erythromycin, either taken orally or applied topically

Brain Dev (1992) 14(3):1704