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These data demonstrate that high glucose induces PSC via activation of the TGF/SMAD pathway

We next asked whether reactive oxygen species (ROS) or subsequent NRF2 induction caused by GSH depletion could impact SLC25A39 levels

To explore the role of RRM2 in the PI3K/Akt signaling pathway, we examined the expression levels of PI3K pathway-related proteins including Akt, phosphorylated Akt (pAkt), PI3K kinase p85, PI3K kinase p110, and PTEN in TPC-1, RRM2 knockdown ( RRM2 -KD-TPC-1), and RRM2 overexpression ( RRM2 -OE-TPC-1) cells by Western blot