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The BL21 transformants were plated on LB agar plate with kanamycin (KAN) (30 g/mL) and incubated overnight at 37 C

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Potential Long-Term Risks Although AOD-9604 does not increase IGF-1 levels, there are concerns about its long-term metabolic effects, including: Unknown impact on fat metabolism regulation Since AOD-9604 alters fat-burning pathways, researchers are still investigating whether prolonged use could affect natural fat metabolism

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