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In this review, we focus on three specific methods that have been used to study the neuroimmunology of ME/CFS: positron emission tomography (PET) using translocator protein (TSPO) binding radioligand, magnetic resonance spectroscopy (MRS), and assays measuring cytokines in blood and cerebrospinal fluid We offer a particular focus on what can and cannot be concluded by studies using these methods

Calcium mobilization assay Calcium mobilization assay to measure GHSR signaling was carried out in HEK-293T cells cultured in DMEM supplemented with 10% (vol/vol) FBS (Fisher Scientific)

Oncol Rep (2013) 29(2):6859

To stain lipids, 1 g/mL Nile Red (AAT Bioquest, USA) was added to the medium, and cells were incubated at 37 C for 15 min, then washed with PBS and fixed