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An immunohistochemical approach by triple staining of VGLUT1, VGLUT2, and an SV marker synaptophysin (Syp) revealed that although substantial populations of vesicle clusters are dominated by one of the two VGLUT isoforms, the majority of Syp-positive presynaptic structures contain both isoforms at different levels, consistent with other studies (Billups, 2004), there are no alterations in evoked EPSC amplitudes, or in the frequency and amplitude of miniature EPSCs in VGLUT1-deficient calyx of Held synapses (Nakakubo et al., Drosophila neuromuscular junctions in which gradual reduction of the ortholog, DVGLUT, results in a reduction of mini frequencies, but not mini amplitudes, arguing that a single copy of VGLUT on an SV suffices to fill up SVs completely (Daniels et al., 2004

Use the gauze to put light pressure on the site

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46 , 938 (1960) Knox, E.: Glutathione

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