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Validation of Zebrafish (Danio rerio) Reference Genes for Quantitative Realtime RTPCR Normalization

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The fertilization rate (the number of 2PNs divided by the total number of MII oocytes), quality of obtained embryos, implantation rate (the number of intrauterine gestational sacs divided by transferred embryos), as well as clinical pregnancy rate (by detecting fetal heartbeats two weeks following the positive hCG) and ongoing pregnancy rate (a pregnancy documented by ultrasound over 20 gestational weeks that showed the presence of normal fetus)

Dysfunction of the carnitine transporter OCTN2 is the cause of Primary Carnitine Deficiency (PCD), which is characterized by systemic loss of carnitine with severe clinical manifestations, including fertility issues in both males and females

The only way to increase carnitine in muscle is very prolonged carnitine supplementation in conjunction with a very high carbohydrate load or any other feeding that produces high insulin levels