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Only upon addition of substrates for transhydrogenase forward activity, a change in the signal is observed for the wt enzyme, implying that the transhydrogenase proton translocation channel is gated (by the nucleotides binding sites) to prevent short circuiting of protons and thus loss of pmf

Sakurama H, Kiyohara M, Wada J, Honda Y, Yamaguchi M, Fukiya S, et al

The assay conditions were used for the screening assay and each drug compound was tested at 8 different concentrations (M) starting at 30 M final assay concentration with a half-log dilution and 2 replicates per concentration

resulting in apoptotic markers of oxidative stress which have been demonstrated in lymphocytes of diabetics with chronic non-healing wounds, with reduced levels of catalase and glutathione 39