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Similarly, a large hydrophobic region behind the active site of PDI was demonstrated to have an important role in its activity

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It plays a key role in: Neutralising free radicals Supporting liver detoxification Protecting skin cells from oxidative damage Maintaining even skin tone and clarity However, factors like pollution, stress, aging, poor diet, and UV exposure can reduce glutathione levels which is why supplementation is often recommended

9 Future directions and challenges Although substantial progress has been made in elucidating the molecular interplay between ROS, mitochondrial dynamics, calcium signaling, and antioxidant defense systems in tendon biology, several conceptual and translational challenges remain