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10.1016/j.wneu.2015.09.052 61 GuoS.XingN.XiangG.ZhangY.WangS

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These ingredients were selected for their roles in supporting tyrosinase balance, antioxidant defense, visible tone refinement, and the reduction of oxidative stress associated with persistent pigmentation concerns

Cells were fixed in 4% (v/v) paraformaldehyde (PFA) (Pierce, 28906) in PBS for 20 min and permeabilised in 0.1% (v/v) Triton X-100 in PBS (Sigma-Aldrich), or in 0.1% (w/v) saponin (sigma-Aldrich) in PBS when labelling LAMP1 compartments, for 5 min followed by blocking with 2% (w/v) BSA (Sigma, 05482) in PBS for 30 min

King, head laboratory scientist