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The absorption at 420 nm and 550 nm of the supernatant was measured against a mixture of 100 L lysis buffer, 900 L resuspension buffer, 100 L 0.4 % pNPP solution and 200 L stop solution in a BioSpectrophotometer (Eppendorf, Hamburg, Germany)

Plant Biotechnol J 15:271284

Glutamine protection in an experimental model of acetaminophen nephrotoxicity

Under physiological conditions, a small proportion of electrons (approximately 2%) prematurely leak from the ETC prior to reaching complex IV, leading to the partial reduction of oxygen and the formation of superoxide [78]