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Cells were lysed by probe sonication in PBS and protein concentrations were measured by a bicinchoninic acid (BCA) assay and normalized to 4 mg/ml

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then, a cyclic RGD peptide, which selectively recognizes v 3 and v 5 integrin receptors, conjugated adamantine was anchored on the surface of the -CDP-based nanomicelle to form a TPA bionanoprobe (DEASPI/ -CDP@RGD) by the -CD/adamantine host-guest inclusion strategy (Figure 4B)

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