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The cells were mechanically disrupted using cycles of vortexing (60 seconds) and ice cooling (120 seconds) for a total of 10 cycles

Trends Mol Med 19: 418427, 2013 [DOI] [PubMed] [Google Scholar] 164.Lopez M, Varela L, Vazquez MJ, Rodriguez-Cuenca S, Gonzalez CR, Velagapudi VR, Morgan DA, Schoenmakers E, Agassandian K, Lage R, Martinez de Morentin PB, Tovar S, Nogueiras R, Carling D, Lelliott C, Gallego R, Oresic M, Chatterjee K, Saha AK, Rahmouni K, Dieguez C, Vidal-Puig A

J Cell Biol (2013) 202:95166

Transcripts per million (TPM) generation and differential expression analysis was performed on aligned reads to Mus musculus UCSC GRCm38/mm10 reference genome using Illumina RNA-seq differential expression software (DESeq2, v1.0.1) 54

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