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It is thought that stages 2 and 3 should be targeted with prophylactic therapies, such as lipid lowering drugs or RAS modifiers [13], to minimize the progression of CKD

Suzuki T, Shibata T, Takaya K, Shiraishi K, Kohno T, Kunitoh H, Tsuta K, Furuta K, Goto K, Hosoda F, Sakamoto H, Motohashi H, Yamamoto M (2013) Regulatory nexus of synthesis and degradation deciphers cellular Nrf2 expression levels

If the test reaffirms a positive outcome, the sample undergoes gas chromatography/mass spectrometry to pinpoint the specific substance in the urine

Investigation findings

For the closely eluting VVVFIKPTC(GSH)PYC(O3)R and VVVFIKPTC(O3)PYC(GSH)R peptides, the abundance was determined by summing the area of the XIC of the 3 transitions that are unique to the peptides (VVVFIKPTC(GSSG)PYC(O3)R: m/z 939.433 ++ 326.113 [y2] + ,C(GSH), 489.176 [y3] + Y, and 586.229 [y4] + P, and VVFIKPTC(O3)PYC(GSH)R: ++ 538.196 [y2] + C(O3), 746.260 [y3] + Y, and 843.312 [y4] + P) (diagnostic ions are boxed in the ion tables in Supplementary Fig