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One aliquot was analysed immediately with a Spincell 3 haematology analyser (Monlab), while others were centrifuged at 23,000 g for 10 min at room temperature in a Sigma 112 mini centrifuge to obtain plasma

3 Network analysis of host immune-inflammatory pathways and gut microbiota perturbations The intricate interplay between the gut microbiota and the hosts immune-inflammatory pathways is pivotal in maintaining endocrine homeostasis, particularly concerning thyroid function