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Therefore, this type of linker can remain stable in the blood system while releasing active payloads exclusively in cancer cells with elevated GSH levels

Bostrm P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, Rasbach KA, Bostrm EA, Choi JH, Long JZ, et al

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To imitate the architecture of branched ubiquitin chains conjugated to OTUD5, i.e., distal K48 linkages branching off the proximal K29 linkages, we inserted monoubiquitins into the four K48 residues within a K29-linked tetraubiquitin, resulting in a K29/K48-linked branched octamer (Fig