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NADPH solution was added to the plates, and the absorbance was measured at 412 nm using a CLARIOstar microplate reader (CLARIOstar, BMG LABTECH, Germany)

Methods: Female, C57BL/6J mice aged 12-14 weeks received Lieber-deCarli diet with alcohol for 10 days followed by one binge (AH model) or calorie matched control diet

Mitochondrial Support ALA may support normal mitochondrial function and the bodys natural cellular energy processes

At OD 600 0.8, gene expression for the POI was induced by further adding 0.5 mM IPTG (from a 0.5 M stock solution in ddH 2 O) and continued for 16 h (cytoplasmic expression) or 3 h (periplasmic expression) at 30 C

These proteins could potentially support the absorption of glutathione precursors, such as cysteine, which is crucial for glutathione synthesis