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Images of stained cells were captured using a Leica DMi8 inverted live-cell microscope under ultraviolet light excitation (385 nm) to detect live cells with fluorescent blue nuclear stain

Moreover, leonurine exerts potent cardioprotective effects by either increasing the level of p-PIK, p-AAKT, p-GSK3 and Bcl-2, or decreasing the levels of caspase 3, cleaved-caspase3 and Bax (Xu et al., 2018)

Funding This publication is solely financed by the Wroclaw University of Environmental and Life Sciences

FIGURE 3 3.3 Effects of FEV knockdown on cellular senescence and ferroptosis To further clarify whether FEV affects HUVEC senescence through ferroptosis, the researchers used RNA interference (RNAi) to knock down the expression of FEV (Figure 4A), and then assessed the senescence by -gal staining

Desikan SP, Ravandi F, Pemmaraju N, Konopleva M, Loghavi S, Jabbour EJ, Daver N, Jain N, Chien KS, Maiti A, Montalban-Bravo G, Kadia TM, Macaron W, DeLumpa R, Kwari M, Borthakur G , Short NJ